Manuscript Discussion Section Essay Example | Topics and Well Written Essays - 500 words

Manuscript Discussion Section - Essay ExampleIn this study affinity chromatographic technique has been used to derive fecundation affinities. With the application of affinity chromatography to direct determination of functional activities, there is problem with determination of functional activities, indicating the fact that affinity in binding may non be directly correlated with the pharmacological action of the drug on the pharmacological target. In case of competitive agonists and antagonists, these properties are related using Cheng-Prusoff relationship,where Ki is the binding affinity of the inhibitor, IC50 is the functional strength of the inhibitor, S is substrate concentration, and Km is the affinity of the substrate for the enzyme. The Cheng-Prusoff relationship cannot be used with NCAs due to occurrence of allosteric interactions. The carnal knowledge antagonistic activities of a series of noncompetitive antagonists, namely, imipramine, ethidiuum, phencyclidine, dextrome thorphan, and mecamylamine expressed as IC50 values towards nAChRs can be measured using data from affinity chromatography on an immobilized nAChR nonmoving phase using nondirect method of multivariate analysis for assessment of IC50 values.